Optimizing a bioprocess requires timely insight into both product quality and the conditions that drive it. From understanding critical quality attributes (CQAs) of biotherapeutic proteins to monitoring how cell culture media composition and feeding strategies impact performance, analytical workflows play a central role in decision-making.

Traditional mass spectrometry workflows can introduce complexity through sample preparation, method development, and longer analysis times—creating delays between sampling and actionable insight.

This webinar explores a streamlined approach to mass spectrometry workflows using capillary electrophoresis-based separations to enable faster, simplified analysis across both protein characterization and cell culture media. Attendees will see how high-resolution separations and direct MS interfacing can support rapid charge variant and glycoform profiling, while also enabling analysis of small, polar metabolites and media components from the same platform.

Through real-world examples, including quantitative analysis of key metabolites in cell culture media using established assay kits, we will demonstrate how these workflows can reduce sample preparation, shorten time to results, and provide reproducible, quantitative data to support bioprocess development.

Learning Objectives:

• Understand how a microfluidic, direct-to-MS interface simplifies mass spectrometry workflows, eliminating columns, gradients, and complex setup while enabling high-resolution separations
• See how streamlined workflows reduce sample preparation and analysis time, accelerating biotherapeutic protein characterization and enabling faster, more informed decision-making
• Explore real-world data from case studies showing how a unified MS workflow supports both protein and cell culture media analysis, including quantitative insight into key metabolites that impact bioprocess performance

Speakers

Hampus Engstroem

Repligen
Field Application & Marketing Manager

Hampus Engstroem is a Field Applications & Marketing Manager at Repligen, where he supports and drives adoption of the ZipChip® CE-MS and REBEL® XT Media Analyzer platforms. He brings over a decade of hands-on experience in mass spectrometry and molecular biology, with a strong focus on translating complex analytical technologies into practical, high-impact workflows for biopharma and research applications.

Prior to joining Repligen, Hampus held multiple roles at 908 Devices, including Field Application Scientist, Team Lead for the North American Field Applications organization, and Product Management Liaison for the ZipChip CE-MS product, where he played a key role in customer support, product development feedback, and market positioning. Earlier in his career, he managed the George L. Wright Jr. Center for Biomedical Proteomics core at the Leroy T. Canoles Jr. Cancer Research Center at Eastern Virginia Medical School, overseeing advanced proteomics workflows and supporting translational cancer research.

Scott Mellors, Ph.D.

Move Analytical
Founding Partner and Science Lead

Dr. J. Scott Mellors is Founding Partner and Science Lead at Move Analytical, where he leads production efforts as well as the translation of research ideas into practical customer tools. With over two decades of experience in analytical chemistry and instrumentation, Dr. Mellors is widely recognized as an expert in capillary electrophoresis-mass spectrometry (CE-MS) and ultrahigh-pressure liquid chromatography (UHPLC).

Scott earned his Ph.D. in Chemistry from the University of North Carolina at Chapel Hill in 2005, conducting research on UHPLC under the mentorship of Professor James Jorgenson. He subsequently joined the laboratory of Professor J. Michael Ramsey as a staff scientist, focusing on integrating microfluidic separations with mass spectrometry. During his tenure, he led the microchip electrospray ionization (ESI) subgroup, contributing to advancements in microfluidic CE-MS systems.

In 2014, Scott joined 908 Devices as a Principal Scientist, where he played a pivotal role in the development of the ZipChip® and REBEL platforms. These innovations have enhanced analytical workflows by providing rapid, high-resolution separations with minimal sample preparation. His work has been instrumental in advancing CE-MS technology for antibody characterization and metabolomics applications.